金花柊叶形态特征

目的:建立高效液相色谱法测定大叶金花草中牡荆素含量。方法:采用Waters Symmetry C18(250 mm×4.6 mm,5 μm)色谱柱,流动相为乙腈-0.1%冰乙酸溶液(27∶73),流速为1.0 ml·min-1,检测波长为339 nm,柱温为30℃。结果:牡荆素浓度在10.03~90.27 μg·ml-1范围内与峰面积的线性关系良好(r=0.9999),平均回收率为100.48%,RSD为0.98%。结论:本分析方法准确、灵敏、重现性好,可用于大叶金花草中牡荆素的含量测定。

A RAPD marker specific to leaf shape of fig(Ficus carica)was developed and converted into SCAR marker for germplasm identification.RAPD amplifications with random primers S_ 2082 produced a unique fragment linking to the non-cordate base leaf shape.The unique fragment was extracted,cloned and sequenced.Sequencing results indicated that the size of the fragment was 1 640 bp.Specific primers were designed based on its sequence and used in SCAR-PCR,and it was showed that the RAPD marker from S_ 2082-1640 was successfully converted into SCAR marker.